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Optical sectioning with HMC

Hoffman Modulation Contrast is particularly helpful in selecting 

a plane of observation. The image of a shallow plane of focus 

is not obscured by information above or below this plane. 

Relatively thick specimens can be viewed as series of successive planes.

 

Ideal when using plastic chambers & other birefringent materials.
HMC's ability to produce three-dimensional appearing images through

 birefringent plastic chambers has assisted scientists in gathering exciting 

new information about live specimens grown in plastic culture chambers. 

See photograph 1.

 

 

HMC objectives and condensers available in standard, 

long and extra-long working distance configurations.
The microscopist can look at specimens using a long working distance 

(LWD) condenser of a high numerical aperture (.5NA at 40mm). This 

enables the precise placement of micromanipulators. Other studies 

requiring LWD objectives and condensers such as cardiovascular studies, 

fertility and electrophysiology can be done with ample working space. 

The image will have both high contrast and high resolution. 

See photograph 2.

 

Optical sectioning with HMC
Hoffman Modulation Contrast is particularly helpful in selecting a plane 

of observation. The image of a shallow plane of focus is not obscured by 

information above or below this plane. Relatively thick specimens can

 be viewed as series of successive planes. 

See photographs 3a, b.

 

Can visualize structures which have been labeled with Fluorescence.
A transmitted modulation contrast microscope can be used in combination 

with incident light fluorescence. The morphology of fluorescing and non-fluorescing 

structures will be revealed without changing the objective or moving the specimen. 

See photographs 4a, b.

 

Eliminates halo common to phase contrast.
Phase contrast produces a halo effect. In some cases, the halo can obscure

 important information. Modulation Contrast does not create a halo. It therefore 

permits precise viewing of minute structural details such as edge configuration 

and lends itself to metrology. 

See photographs 5a, b.

 

 

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